The first half minute of the episode shows the Cheerios in their rehearsal stunts and Sue Sylvester's first of many punch lines -
"You think this is hard? Try being waterboarder, that's hard!". It closes with the Journey song that the Glee cast popularized - Don't Stop Believing.
Being a starter episode, this also acquaints us with each character:
Will Scheuster, the Spanish teacher and Glee Club coach.
Sue Sylvester, the notorious cheerleading coach.
Rachel Berry, the ambitious Glee club member who cared too much about living her high school life to the fullest by being popular.
Finn Hudson, the charming quarterback who is being coveted by all girls (and, well..a few boys).
Quinn Fabray, the head cheerleader who was also Finn's girlfriend.
Artie Abrams, the nerd in a wheelchair with a cool singing voice.
Tina Cohen-Chang, the goth girl who pretends to stammer.
Noah 'Puck' Puckerman, the all-time school bully who could actually sing and is charming when he isn't being such a bully.
Santana Lopez, the hot latina cheerleader who could sing
Brittany Pierce, the cheerleader whom anybody could label as the dumb blond. One could easily say she's the best dancer in the show.
Ken Tanaka, the football coach who is in love with the school guidance counselor.
Emma Pilsbury, the school guidance counselor who is in love with Will Scheuster.
Terry Scheuster, Will's sorta crazy wife. Or crazy-in-love wife.
Figgins, the school principal who cares too much about budgeting the school's financial resources more than anything.
Okay, so much for the introduction of characters. I don't wanna give any more spoilers than I already have. Download Season 1 Episode 1 from the links below here and see for yourself.